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Answer:
1 can be correctly matched with A, Calcium chloride enhances the permeability of plasmid DNA within the cell.
2 can be correctly matched with B, only the ampicillin-resistant cells or the transformed cell possesses the tendency to grow in the selective media.
3 can be correctly matched with the pGLO plasmid, the isolation of GFP was done originally from jellyfish, and is usually administered within the pGLO plasmid for examining the expression.
4 can be correctly matched with D, the expression of GFP is instigated by arabinose as a bidirectional promoter is shared by GFP with the arabinose gene, and thus can get stimulated in its existence.
5 can be correctly matched with E, in the presence of ultraviolet light, the green fluorescent protein gets fluorescent.
The correct match for the given Function in a transformation experiment
- 1 (A) Cacl2 increases the permeability by dragging the DNA along with it into the cell.
- 2 (B)Only ampicillin-resistant (transformed) cells can grow in that selective media.
- 3 (C) GFP was isolated originally from jellyfish and is generally inserted in the Palo plasmid for expression analysis.
- 4 (D) Arabinose induces GFP expression because GFP shares a bidirectional promoter with the arabinose gene and hence can be induced in its presence.
- 5 (E) The GFP fluoresces under UV light, but long exposure can damage DNA.
Function in a transformation experiment
the plasmid DNA, or pGLO, contains a gene for Green Fluorescent Protein (GFP) as well as the gene for resistance to the antibiotic ampicillin.
Under UV lights for a longer duration can damage the DNA, however, it fluoresces GFP. GFP shares a bidirectional promoter with the arabinose gene hence it is induced in its presence.
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